A simple, economic and accurate method was developed for the simultaneous determination of clotrimazole and lignocaine HCl in ear drops by RP-HPLC. Chromatographic separation of the drugs, achieved on Hypersil C-18 (250×4.6 mm, 5μ) column at 225 nm, mobile phase comprising a mixture of methanol-buffer in the ratio of 90:10 v/v, at a flow rate of 1.1 mL/min. Lignocaine HCl and clotrimazole were eluted at 3.382 and 4.2 mins respectively. Calibration curves were linear over the range of 10-250 μg/ml, 10-300 μg/ml for clotrimazole and lignocaine HCl with r2 values, 0.997 and 0.999 respectively. The % assay of clotrimazole and lignocaine HCl were 99.6 and 98.98% respectively. The performance of the method was validated according to ICH guidelines. The proposed method was successfully applied for the determination of clotrimazole and lignocaine HCl in ear drops. The performance of the method was validated according to ICH guidelines.
Loading....